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SCLC study MGH - WES dataset

Dataset ID Technology Samples
EGAD00001003970 Illumina HiSeq 2000 7

Dataset Description

For whole-exome sequencing 1 µg of DNA from fresh-frozen tumors was fragmented by sonication technology (for DNA from fresh-frozen tumors: Bioruptor, diagenode, Liѐge, Belgium; for DNA from FFPE material: Covaris). The fragments were end-repaired and adaptor-ligated, including incorporation of sample index barcodes. After size selection, libraries were subjected to an enrichment process with Sure select XT (Agilent). The final libraries were sequenced with a paired-end 2×75 bp protocol for an average coverage of 100-120x