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Single-cell RNA sequencing of cytokine-induced T cell responses

Dataset ID Technology Samples
EGAD00001005290 Illumina HiSeq 4000 16

Dataset Description

Cytokines affect T cell responses by polarising them to different phenotypes. We isolated T cells from healthy platelet donors and cultured them in resting and stimulated condition, as well as in the presence of Th2, iTreg and Th17 polarizing cocktail. To characterize the efficacy of cytokine induced porization and subpopulation specific response, we profiled single cell transcriptome five days following polarization using 10x platform (3' v2).

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