DAC

DAC for HTS MRD study

Dac ID Contact Person Email Access Information
EGAC00001000359 Jack Bartram j [dot] bartram [at] ucl [dot] ac [dot] uk No additional information is available

This DAC controls 1 dataset:

Dataset ID Description Technology Samples
EGAD00001001983 Immunoglobulin heavy chain gene high throughput sequencing of paediatric acute lymphoblastic leukaemia samples, for the purpose of MRD on the Illumina MiSeq platform. This dataset contains summary fastq files and raw bcl files from the MiSeq for this study. In the study we identify errors associated with multiplexing that could potentially impact on the accuracy of MRD analysis. We optimise a strategy combining high purity, sequence-optimised oligonucleotides, dual-indexing and an error-aware demultiplexing approach to minimise errors and maximise sensitivity. Illumina MiSeq; 491