DAC for Sardinia Leukocytes polyA RNAseq 624 project

Dac ID Contact Person Email Access Information
EGAC00001000561 Francesco Cucca francesco [dot] cucca [at] irgb [dot] cnr [dot] it No additional information is available

This DAC controls 1 dataset:

Dataset ID Description Technology Samples
EGAD00001003102 We sequenced the polyA+ fraction of the RNA of the leukocytes from 624 sardinian individuals with RNAseq. Prior to library preparation we added either ERCC ExFold RNA Spike-In. An average of 60M reads per samples with 51 bp paired-end reads were generated on a HiSeq 2000 (Illumina). Sequencing reads were then aligned using STAR-2.2.0c2 to the h37d5 reference genome supplemented with the ERCC spike-ins sequences. We further provided an exon-exon junction database that we generated from the GENCODE v14 annotation. In order to remove a contamination from a parallel experiment, we discarded any reads that mapped to the genomic regions of CBLB (chr3:105370773-105592330) and BCL11A (chr2:60672555-60784156). Filtered aligned reads (bam format) are shared. Illumina HiSeq 2000 624