DAC

PUMCH-ICT Hepatocellular Carcinoma Study Data Access Committee

Dac ID Contact Person Email Access Information
EGAC00001000628 Haitao Zhao zhaoht [at] pumch [dot] cn No additional information is available

This DAC controls 1 dataset:

Dataset ID Description Technology Samples
EGAD00001003397 Twenty samples were collected in pairs, i.e., HCC tissue and adjacent non-cancerous tissue. The collected tissue samples were stored in liquid nitrogen. First, 50 mg of tissue was lysed in TRIzol (Invitrogen) to extract RNA following the manufacturer’s instructions. Next, ribosomal RNA was depleted using a RiboZero Gold kit (Epicentre Bio-technologies). RNA integrity was assessed with an Agilent Bioanalyzer 2100. An RNA-Seq library was generated with the rRNA-depleted samples using an Illumina standard RNA Sample Prep kit according to the manufacturer’s instructions. The library was subsequently sequenced on an Illumina HiSeq2500 as 125-bp paired-ends with approximately 300-bp size selection. Illumina HiSeq 2500 20