DAC
Oxford Molecular Diagnostic Center DAC
| Dac ID | Contact Person | Access Information | |
|---|---|---|---|
| EGAC00001000866 | Anna Schuh | anna [dot] schuh [at] oncology [dot] ox [dot] ac [dot] uk | No additional information is available |
This DAC controls 1 dataset:
| Dataset ID | Description | Technology | Samples |
|---|---|---|---|
| EGAD00001004047 | Peripheral blood mononuclear cells (PBMC) of CLL patients were isolated by density-gradient centrifugation over Linfosep (Biomedics, Madrid, Spain). B cells were purified with a CD19+ magnetic-bead system (MidiMACS, Miltenyi Biotec, Bergish Gladbash, Germany) according to the manufacturers’ instructions. Mean B-cell purity was >99% and the mean percentage of CD5+/CD19+ cells after purification was >98%, as measured by flow cytometry. Total RNA was extracted from purified cells in a single step using TriPure Isolation Reagent (Roche Applied Science, Vilvoorde, Belgium).Whole transcriptome sequencing libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina). Libraries underwent 2 × 76 bp paired-end sequencing on a HiSeq 2500 instrument (Illumina). The median number of paired-end reads was 60.5 million (range, 49.7-79.7 million). | Illumina HiSeq 2500 | 32 |
