DAC
AREP(Association pour la Recherche et l’Enseignement en Pathologie) Data Access Commitee
| Dac ID | Contact Person | Access Information | |
|---|---|---|---|
| EGAC00001001196 | Guillaume Assie | guillaume [dot] assie [at] aphp [dot] fr | No additional information is available |
This DAC controls 3 datasets:
| Dataset ID | Description | Technology | Samples |
|---|---|---|---|
| EGAD00001004996 | Total RNA was extracted using RNAble (Eurobio), cleaned-up with RNeasy columns (Qiagen) and sequenced. The libraries were prepared at the Genomics Platform of the Cochin Institute, following the TruSeq Stranded mRNA protocol (Illumina), starting from 1 µg of high quality total RNA. Paired end (2 × 75 bp) sequencing was performed on a Nextseq 500 platform (Illumina). FASTQ sequences were aligned on hg19 (GRCh37) human reference genome with STAR (v.2.5.2a) | NextSeq 500 | 134 |
| EGAD00001004997 | Whole‐exome sequencing was performed using NimbleGen MedExome capture (Roche NimbleGen, Madison, WI, USA) from 1 μg of high quality genomic DNA, followed by sequencing of libraries using paired-end mode (2x 75bp) on a Nextseq 500 platform (Illumina, San Diego, CA, USA), at the Genomics Platform of the Cochin Institute. Reads were aligned on hg19 (GRCh37) using BWA V0.7.17. | NextSeq 500 | 86 |
| EGAD00001004998 | Small RNA (<100 bases in length) were purified from total RNA using miRNeasy kit (Qiagen), then sequenced. Libraries were prepared at the Genomics Platform of the Cochin Institute, following the TruSeq small RNA protocol (Illumina), starting from 1 µg of high quality total RNA. Single read (1 × 75 bp) sequencing was performed on a Nextseq 500 platform (Illumina). FASTQ sequences were aligned on miRBase v.2052, then counted with STAR (v.2.5.2a). | NextSeq 500 | 111 |
