DAC for TCR-seq analysis of human intestinal Trm cells

Dac ID Contact Person Email Access Information
EGAC00001001219 Frode L. Jahnsen f [dot] l [dot] jahnsen [at] medisin [dot] uio [dot] no No additional information is available

This DAC controls 2 datasets:

Dataset ID Description Technology Samples
EGAD00001005049 5000 cells of each subset of CD8 T cells (CD103-KLRG1+, CD103-KLRG1- and CD103+ from LP and CD103+ IELs) were sorted into tubes. A modified SMART protocol was used in first-strand cDNA synthesis, and TCRalpha / TCRbeta genes were amplified in two rounds of semi-nested PCR reaction, following the method described in detail in Risnes et al., 2018. Illumina MiSeq 40
EGAD00001005050 Single-cell TCRalpha-beta sequencing of LP CD103+ CD8 T cells from the grafted/native duodenum of two donors (Ptx#1 and Ptx#2) before and 1 year after transplantation. Single cells were sorted into 96-well plates. Paired TCRalpha and TCRbeta sequences were obtained after three nested PCR with multiplexed primers covering all TCRalpha and TCRbeta V genes, as described before (Risnes et al., 2018), and original protocol in (Han et al., 2014). Illumina MiSeq 6