DAC

Placeholder DAC

Dac ID Contact Person Email Access Information
EGAC00001001788 Natalie Stickle natalie [dot] stickle [at] uhn [dot] ca No additional information is available

This DAC controls 1 dataset:

Dataset ID Description Technology Samples
EGAD00001006555 To investigate the mechanism by which GATA1s and STAG2 deficiency contribute to Down Syndrome leukemogenesis, specifically within the propagating CD34/CD117 cell fractions from primary xenografts, we carried out transcriptional and epigenetic profiling by RNAseq and ATACseq. The chromatin accessibility landscape was compared to bulk ATACseq of individually sorted N-FL HSPC subpopulations. To investigate the mechanism underlying the synergy between T21 and GATA1s in driving preleukemia development, we analyzed the binding occupancy of GATA1. We performed Cut&Run assays to profile genome-wide GATA1 binding sites and also to quantify binding changes upon GATA1s editing in N-FL and T21-FL CD34+ enriched HSPCs. Lastly, we profiled miRNAs from N-FL and T21-FL CD34+ enriched HSPCs by miRNA-Seq. Illumina HiSeq 2500,NextSeq 500 91