DAC

RCI Regulatory Tcell DAC

Dac ID Contact Person Email Access Information
EGAC00001001865 Nicholas Strieder nicholas [dot] strieder [at] ukr [dot] de No additional information is available

This DAC controls 6 datasets:

Dataset ID Description Technology Samples
EGAD00001006779 T cells were isolated from human blood and tissues: skin and fat. Subsequently, CD4+ T cells and CD25+ T cells were FACS sorted. scATAC libraries were prepared using the 10x Genomics Kit (CG000168_ChromiumSingleCell_ATAC_ReagentKits_UserGuide_RevD.pdf) and sequenced on an Illumina NextSeq550. In total 15 samples were prepared NextSeq 500 15
EGAD00001007660 Treg and Tfh cells (8 Samples)from the same donors was subject to ATAC-seq processing. Paired end fastq-files are supplied. NextSeq 550 8
EGAD00001007661 Regulatory Tcells were FACS sorted and processed with 10x Genomics Chromium Next GEM SingleCell V(D)J Reagents Kits v1.1 sequencing. In total 17 samples were processed. Fastq files are supplied. NextSeq 550 17
EGAD00001007662 Treg and Tfh cells (8 Samples)from the same donors was subject to RNA-seq (TruSeq Stranded Total RNA) processing. Single end fastq-files are supplied. NextSeq 550 8
EGAD00001007663 Tcells were isolated fom Tissues and FACS sorted. In total 50 samples were processed in 5 replicates with the Takara SmartSeq Stranded kit. Single end fastq-files are supplied. NextSeq 550 50
EGAD00001007664 Tcells were isolated fom Tissues and FACS sorted. In total 43 samples were processed in 5 replicates with the Takara SmartSeq Stranded kit. Single end fastq-files are supplied. NextSeq 550 43