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BioMed X EAC Data Access Committee

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Thomas Rückle
tr@bio.mx

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This DAC controls 6 datasets

Dataset ID	Description	Technology	Samples
EGAD00001009405	Primary lung fibroblast were isolated from well-matched control donors (no COPD, n=3) and patients with COPD (GOLD stage I-IV, n=8). Total RNA of cultured human lung fibroblast were isolated at passage 3 and rRNA was depleted. 75 bp single-end reads were generated from RNA libraries on Illumina NextSeq 500.	NextSeq 500	11
EGAD00001009406	Primary lung fibroblast were isolated from well-matched control donors (no COPD, n=3) and patients with COPD (GOLD stage I-IV, n=8). Genomic DNA of cultured human lung fibroblast was isolated at passage 3, fragmented by tagmentation, and subjected to bisulfite treatment. 100 bp paired-end reads were generated from DNA libraries on Illumina HiSeq2500 platform.	Illumina HiSeq 2500	11
EGAD00001010251	Single-cell RNA seq data of epithelial cells ((EpCAM-enriched by FACS) isolated from cryopreserved human lung tissue (3 samples, 3 donors)	NextSeq 550	3
EGAD00001010252	Bulk RNA seq data from human lung fibroblasts isolated from fresh and cryopreserved lung tissue (18 samples, 3 donors)	NextSeq 500	3
EGAD00010002463	EPIC Array data from human lung fibroblasts isolated from fresh and cryopreserved lung tissue (16 samples, 3 donors)	Illumina_EPIC	16
EGAD50000000018	SLE serum stimulation induced a unique expression profile in human colon organoids marked by a reduction in goblet cell marker expression and mucus composition. Transcriptomic analysis of SLE human colon biopsies displayed a downregulation of epithelial secretory markers. Collection of raw sequencing data used in this publication https://doi.org/10.1101/2023.07.04.547690	Illumina NovaSeq 6000	64