Male Germ Cell Data Access Commitee

Dr Sandra Laurentino
sandra.laurentino@ukmuenster.de

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Dataset ID	Description	Technology	Samples
EGAD00001008652	To study global transcriptional dynamics during human spermatogenesis we sequenced total RNA of human testicular biopsies with 5 specific histological phenotypes: Sertoli cell-only (SCO, n=3), spermatogenic arrests at the spermatogonial (SPG, n=4), spermatocyte (SPC, n=3), and spermatid (SPD, n=3) level, as well as normal spermatogenesis (Normal, n=3).	NextSeq 500	16
EGAD50000001677	This dataset consists of trancriptome sequencing of human testicular samples with full spermatogenesis but different ages. We evaluated three age groups, namely young (24-31 years old, n=4), middle-aged (41-45 years old, n=3), and aged (54-75 years old, n=6). RNA was isolated from snap-frozen bipsies using Direct-Zol RNA microprep kit and the quality was evaluated with RNA ScreenTape. Samples were rRNA depleted (NEBNext rRNA depletion kit), libraries were prepared (NEBNext Ultra RNA II directional Library Prep kit), and sequenced (2x150 cycles, NextSeq 500). Sequencing files were uploaded as fastq. Sequencing data for samples G1.3 and G1.4 were also used in a previous study (Siebert-Kuss et al., 2022) and are deposited in EGA under accession number EGAS00001006135 as CTR-1 and CTR-3, respectively.	NextSeq 500	11