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LifeLines-NEXT data access commitee

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Alexandra Zhernakova
a.zhernakova@umcg.nl

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This DAC controls 10 datasets

Dataset ID	Description	Technology	Samples
EGAD00001011291	This dataset contains sequencing data from VLP-enriched fecal microbiome from LLNEXT project	HiSeq X Ten	206
EGAD00001011293	This dataset contains sequencing data from fecal microbiome from LLNEXT project	HiSeq X Ten	321
EGAD50000000180	16S v3-v4 amplicon sequencing of milk, fecal and oral cavity samples, along with sequencing controls, of a subset of newborn infants from Lifelines NEXT cohort	Illumina MiSeq	98
EGAD50000000181	16S-ITS-23S amplicon long read PacBio sequencing of milk, fecal and oral cavity samples, along with sequencing controls, of a subset of newborn infants from Lifelines NEXT cohort	Sequel II	66
EGAD50000000531	The dataset contains HMO measurements for 1542 samples from Lifelines NEXT cohort	Illumina MiSeq	1502
EGAD50000000532	This dataset contains 16S sequencing of fecal and milk samples for HMO-microbiome Lifelines NEXT study	Illumina MiSeq	1501
EGAD50000001187	This dataset contains fecal metagenomic sequencing of Lifelines NEXT cohort samples (main batch sequenced until first data freeze, end of 2023).	Illumina HiSeq 2000	4577
EGAD50000002469	This dataset contains metagenomic sequencing (MGS) data derived from vaginal swabs and breast milk samples collected from 173 participants in the Lifelines NEXT cohort. Sequencing was performed using Illumina next-generation sequencing technology, and the resulting sequence reads are provided as FASTA files for downstream microbiome analysis.	Illumina HiSeq 2000	173
EGAD50000002666	Sequencing was performed on the NovaSeq 6000 platform by Illumina using 2 × 150 bp paired-end chemistry. For each fecal sample, three output files were generated: forward paired-end reads, reverse paired-end reads, and unpaired singleton reads. Raw reads underwent a comprehensive quality-control pipeline including adapter trimming, quality filtering, host-origin filtering to remove human-derived reads, error correction, and duplicate read removal.	Illumina NovaSeq 6000	1124
EGAD50000002677	This dataset contains list of basic phenotypes for Lifelines NEXT participants including: - linkage to metagenomics sequencing - Infant ID - family ID - gestational age - parity - maternal age at delivery - maternal smoking history - maternal hypertension at pregnancy - infant sex - place of delivery - delivery mode - infant weight at birth - infant weight growth slope - infant breastfeeding (never/ever) - infant eczema		4577