DAC - A standardised framework for robust fragmentomic feature extraction from cell-free DNA sequencing data

Dr Matthew M Eldridge
matthew.eldridge@cruk.cam.ac.uk

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Dataset ID	Description	Technology	Samples
EGAD00001015535	Fragmentomic features of cell-free DNA represent promising non-invasive biomarkers for cancer diagnosis. However, a lack of systematic evaluation of biases in feature quantification has hindered the adoption of such applications. We compared features derived from whole-genome sequencing of ten healthy donors using nine library kits and ten data-processing routes, and validated them in 1,182 plasma samples from published studies. Our results clarify the variations resulting from library preparation and feature quantification methods. We designed the Trim Align Pipeline and the cfDNAPro R package as unified interfaces for data pre-processing, feature extraction, and visualisation, aiming to standardise multimodal feature engineering and integration for machine learning.	Illumina NovaSeq 6000	82