MS-EBV project

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Dataset ID	Description	Technology	Samples
EGAD00001016594	This dataset includes paired gene expression (GEX), cell surface protein (CSP) and T cell receptor (VDJ) FASTQ files obtained from 21 MS and 19 control participants. Briefly, cryopreserved PBMCs were thawed and stained with DNA-barcoded dCODE-dextamers to identify EBV antigen-specific T cells, together with sample hashtag antibodies compatible with the 10x Genomics 5′ Feature Barcode workflow. Details for hashtag antibodies and multiplex format is available in the manuscript. Following staining, samples were processed using the Chromium GEM-X Single Cell 5′ Gene Expression and V(D)J workflow with Feature Barcode technology, according to the manufacturer’s instructions. Amplified cDNA was used to generate separate 5′ GEX, CSP and VDJ libraries. Indexed libraries were quantified, pooled and sequenced on NextSeq Illumina platform.	NextSeq 500	50