DIAMOND DAC

Ms Charlotte Proudhon
charlotte.proudhon@inserm.fr

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Dataset ID	Description	Technology	Samples
EGAD50000000646	The detection of circulating tumor DNA, which allows non-invasive tumor molecular profiling and disease follow-up, promises optimal and individualized management of patients with cancer. However, detecting small fractions of tumor DNA released when the tumor burden is reduced remains a challenge. We developed a PCR-based targeted bisulfite method coupled to deep sequencing to detect methylation patterns of L1PA elements,which we named DIAMOND (for Detection of Long Interspersed Nuclear Element Altered Methylation ON plasma DNA). We used sodium bisulfite chemical conversion to achieve base-pair resolution analysis and designed a multiplexed PCR based on 8 amplicons covering L1PAs .	Illumina HiSeq 2500 Illumina MiSeq Illumina NovaSeq X	729