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T-bet+ CXCR3+ B cells drive hyperreactive B-T cell interactions in multiple sclerosis

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Dr Roland Martin
roland.martin@uzh.ch

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This DAC controls 5 datasets

Dataset ID	Description	Technology	Samples
EGAD50000001235	10X 3' (RNA) samples of isolated single nuclei from highly inflamed leptomeninges and adjacent grey matter from 3 MS patients used in the paper.	Illumina NovaSeq 6000	6
EGAD50000001236	Methylome and the corresponding transcriptome of autoproliferating/Dim B cells and resting/hi B cells in Multiple sclerosis patients	Illumina NovaSeq 6000	10
EGAD50000001237	10X 3' (RNA) and 10X 5' (VDJ) samples of B cells (non-proliferating/hi and autoproliferating/Dim) for healthy donor and Multiple sclerosis patients used in the paper.	Illumina NovaSeq 6000	126
EGAD50000001269	Methylome of resting/hi and autoproliferating/Dim memory B cells in Multiple sclerosis patients. In brief, non-proliferating (CFSEhi) and AP+ (CFSEdim) CD19+ CD27+ B cells from PBMCs of 6 natalizumab-treated female RRMS (NAT) patients were sorted following 7 days of autoproliferation. Methylome libraries for 12 samples were constructed by applying the post-bisulfite adaptor tagging (PBAT) method on lysed cells and sequencing was conducted on Illumina NovaSeq 6000. CpGs with minimum 10X coverage were used for differential methylation position (DMP) analysis.	Illumina NovaSeq 6000	12
EGAD50000001270	Transcriptional profile of resting/hi and autoproliferating/Dim memory B cells in Multiple sclerosis patients. In brief, non-proliferating (CFSEhi) and AP+ (CFSEdim) CD19+ CD27+ B cells from PBMCs of 5 natalizumab-treated female RRMS (NAT) patients were sorted following 7 days of autoproliferation. Transcriptome libraries were constructed from the same samples, except for one sample pair, which was excluded due to limited input material (5 donors as for Methylome, D1; D2, D3, D5, D6; samples from donor D4 unfortunately technically failed). SMARTer Total Stranded RNA-seq library construction with ribosomal depletion was performed and then sequenced on NovaSeq S4 lane.	Illumina NovaSeq 6000	10