Bulk RNAseq from in vitro generated macrophages and T cells, and mUM tumour biopsies

Dr Adel Benlahrech
adel.benlahrech@immunocore.com

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Dataset ID	Description	Technology	Samples
EGAD50000001225	Tumour biopsies were obtained from uveal melanoma patients (n = 35) pre and 16 days post treatment with tebentafusp. Biopsies, which were either snap frozen or put in RNA later, were analysed by bulk RNA sequencing (50 million reads per sample) using the Illumina NovaSeq system	Illumina NovaSeq X	70
EGAD50000001226	Monocyte derived macrophages were polarised into M1 or M2 using IFNg+ ILP or IL-4 respectively (n =4) . Pan T cells were cultured with or without IL-2 for 4 days (n=4 - 6). Pan T cells were co-cultured with THP1 tumour cells for 20 hours in the presence or absence of ImmTAC molecules and in the presence or absence of M2 macrophages. Sorted T cells and macrophage populations prior and post co-culture were analysed by bulk RNA sequencing (60 million reads per sample) using the Illumina NovaSeq system	Illumina NovaSeq X	52