RNA Sequencing of Control and Myotonic Dystrophy Type 1 Cells During Myogenic Differentiation

Dr Derick G. Wansink
rick.wansink@radboudumc.nl

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Dataset ID	Description	Technology	Samples
EGAD50000001647	To investigate gene expression dynamics during myogenic differentiation in myotonic dystrophy type 1, RNA was collected from immortalized control, patient-derived, and CRISPR/Cas9-corrected myoblasts at proliferation (day -2) and at days 1 and 5 of differentiation. High-quality RNA was isolated and sequenced using poly(A)-enriched or rRNA-depleted protocols, yielding 100 bp paired-end reads with high coverage. A subset of samples at day 1 underwent deeper sequencing to enhance transcriptome resolution.	BGISEQ-500	27