hiPSC scRNA-seq Tremolanti et al.

Dr Ulrika K Marklund
ulrika.marklund@ki.se

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Dataset ID	Description	Technology	Samples
EGAD50000001648	Single nuclei RNA-seq ( 10X Genomics Chromium Gem-X Single Cell 3' Kit v4) of 3 samples of hiPSC lines (cell line KOLF 2.1J iPSC). hiPSC cells were differentiated following a mDA neuron differentiation protocol in three samples: wild type (WT), SULF1/2 DKO and FGF-inhibited (PD/SU treated) SULF1/2 DKO. At day 16, nuclei were isolated from each sample and analyzed with snRNA-seq. Nuclei suspensions from each sample were loaded in different lanes. Reads annotated to chrY (hg38) were discarded from the fastq files following ethical agreements.	Illumina NovaSeq X	3