DAC Magna Græcia University of Catanzaro - WGS of Healthy and PSP Donors

Dr Elvira Immacolata Parrotta
parrotta@unicz.it

Request Access

Dataset ID	Description	Technology	Samples
EGAD50000001758	BAM files of healthy controls (3) and Progressive supranuclear palsy (PSP) patients (4). Genomic DNA from donors was fragmented, end-polished, A-tailed, and ligated with Illumina adapters, followed by size selection. Libraries were PCR-amplified (unless PCR-free), purified with AMPure XP, assessed on the Agilent Fragment Analyzer, and quantified using Qubit and qPCR. Qualified libraries were pooled and sequenced on Illumina platforms, and low-quality reads or adapter-contaminated reads were removed. Clean data were then mapped to the hg38 reference genome using BWA.		7