scRNA-seq of decidual neutrophils at the human maternal fetal interface DAC

Juergen Pollheimer
juergen.pollheimer@meduniwien.ac.at

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Dataset ID	Description	Technology	Samples
EGAD50000002868	This study investigates how neutrophils are locally adapted at the early human maternal-fetal interface and how they contribute to invasive placentation. Using 10x Genomics Chromium Flex single-cell RNA sequencing of first-trimester decidua basalis, together with cytometry, spatial imaging, and functional co-culture assays, we identified decidual fibrinoid-associated neutrophils (dFANs) within the fibrinoid Rohr’s layer. These cells display a compartment-associated phenotype and secrete MMP-9, which promotes extravillous trophoblast motility and contributes to activation of latent TGF-β. The goal of the project was to define the function of neutrophil reprogramming in early pregnancy and its role in supporting human trophoblast invasion.	NextSeq 2000	4