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Oslo University Hospital Data Access Committee for I-BCT study datasets submitted to FEGA Norway

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Dr Olav Engebraaten
olav.engebraten@medisin.uio.no

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This DAC controls 2 datasets

Dataset ID	Description	Technology	Samples
EGAD50000003091	This dataset comprises DNA whole-exome sequencing from a prospective, randomized neoadjuvant trial designed to evaluate DNA-based biomarkers for predicting carboplatin response in patients with early, high-risk breast cancer. The study enrolled 196 patients with with high-risk features, including triple-negative breast cancer (TNBC) and hormone receptor-positive (HR+) breast cancer subtypes. The final analysis included 187 patients, for which WES data is being made available for controlled access. Sample Composition Total samples: 373 (186 blood/normal + 187 tumor) - Blood samples: 186 (germline/reference) - Tumor samples: 187 (tumor tissue biopsies) Technical Specifications - Sequencing platform: Illumina HiSeq4000 - Read type: Paired-end - Read length: 2 × 76 bp - Library preparation: Twist Bioscience Human Comprehensive Exome Plus RefSeq v1 hybrid selection - DNA input: 100 ng per sample - Mean sequencing coverage: 46× for normal samples; 106× for tumor samples	Illumina HiSeq 4000	373
EGAD50000003092	Study Purpose and Design This RNA-seq dataset is derived from the same prospective, randomized neoadjuvant breast cancer trial (IBCT-1) and complements the WXS analysis. RNA sequencing was performed at basline to characterize transcriptomic biomarkers associated with carboplatin response prediction. The cohort includes 187 patients breast cancer tumors across multiple subtypes. Sample Composition - Total samples: 187 (tumor tissue only) Tumor samples: 187 - Sample type: RNA from primary tumor tissue biopsies Technical Specifications - Sequencing platform: Illumina HiSeq4000 - Read type: Paired-end - Read length: 2 × 76 bp - Library preparation: KAPA RNA HyperPrep kit with Twist Bioscience Human Comprehensive Exome Plus RefSeq v1 hybrid selection - RNA input: 100 ng per sample - Sequencing depth: Mean of 35 million read pairs per sample	Illumina HiSeq 4000	187