Genetics of gene expression in primary human immune cells

Study ID Alternative Stable ID Type
EGAS00000000109 Genotype

Study Description

The purpose of this study was to identify cell specific expression quantitative trait loci in freshly isolated highly purified primary B-cells and monocytes from healthy subjects of European ancestry. Cells were purified from Ficoll gradient prepared peripheral blood mononuclear cell fractions using positive selection via magnetic bead labelling of the B-cell marker CD19 and monocyte marker CD14. RNA was extracted from 287 B-cell samples and 288 monocyte samples. RNA was then hybridised to HumanHT-12 v4 BeadChips whole genome expression arrays (Illumina). Individuals were genotyped on HumanOmniExpress-12v1.0 BeadChips (Illumina) at 733202 genetic markers. Data presented include the raw expression values and Robust Spline Normalised values (RSN) for expression for each cell type per individual. Additional genotype data per individual is available on request.

Study Datasets 2 datasets.

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Dataset ID Description Technology Samples
Healthy volunteer collection of European Ancestry
Illumin OmniExpress v1.0 - Illumina GenomeStudio 288
Healthy volunteer collection of European Ancestry
Illumina OmniExpress v1.0-Illumina GenomeStudio 144

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