Study

Analysis of DNA methylation in normal B cells and chronic lymphocytic leukemia

Study ID Alternative Stable ID Type
EGAS00001000534 Other

Study Description

Charting differences between tumors and normal tissue is a mainstay of cancer research. However, clonal tumor expansion from complex normal tissue architectures potentially obscures cancer-specific events, including divergent epigenetic patterns. Using whole-genome bisulfite sequencing of normal B cell subsets, we observed broad epigenetic programming of selective transcription factor binding sites coincident with the degree of B cell maturation. By comparing normal B cells to malignant B cells from 268 patients with chronic lymphocytic leukemia (CLL), we showed that tumors derive largely from a continuum of maturation states reflected in normal developmental stages. Epigenetic maturation in CLL was associated with an indolent gene expression pattern and increasingly favorable clinical outcomes.As second study using these samples focued on evolution and resulting intra-tumor heterogeneity in CLL. We used 450k bead-chip arrays to study DNA methylomes in 68 chronic lymphocytic leukemia (CLL) cases, including 28 serial cases. The result establishs linked evolution of epigenetic and ... (Show More)

Study Datasets 3 datasets.

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Dataset ID Description Technology Samples
EGAD00001001675
RNA-seq of peripheral blood samples from CLL patients.
Illumina HiSeq 2000 42
EGAD00001001676
Tagmentation-based whole-genome bisulfite sequencing of isolated cell types from healthy controls.
Illumina HiSeq 2000 12
EGAD00010000871
CLL and normal B cell samples using 450K
226

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Publications

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