Study

Biased allelic expression in human primary fibroblast single cells.

Study ID Alternative Stable ID Type
EGAS00001001009 Other

Study Description

The study of gene expression in mammalian single cells using genomic technologies now provides the possibility to investigate the patterns of allelic gene expression. We have used single-cell RNA sequencing to detect the allele-specific mRNA level in 203 single human primary fibroblast cells over 133,633 unique heterozygous single nucleotide variants (hetSNVs). We have observed that at the snapshot of analyses each cell contains mostly transcripts from one allele from the majority of genes; indeed 76.4% of the hetSNVs display stochastic monoallelic expression in single cells. Remarkably, adjacent hetSNVs exhibit haplotype consistent allelic ratio; in contrast distant sites located in two different genes are independent of the haplotype structure. Moreover, the allele-specific expression in single cells correlated with the abundance of the cellular transcript. We observed that genes expressing both alleles in majority of the single cells at a given time point are rare and enriched in highly expressed genes. The relative abundance of each allele in a cell is controlled by some ... (Show More)

Study Datasets 2 datasets.

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Dataset ID Description Technology Samples
EGAD00001001083
NA
Illumina HiSeq 2000 2
EGAD00001001084
NA
Illumina HiSeq 2000 209

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