G&T-seq: parallel sequencing of the genomes and transcriptomes of single cells

Study ID Alternative Stable ID Type
EGAS00001001204 Whole Genome Sequencing

Study Description

The ability to simultaneously sequence the genome and transcriptome of the same single cell offersa powerful means to dissect functional genetic heterogeneity at the cellular level. Here we describeG&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from singlecells. By applying G&T-seq to over 220 single cells we reveal cellular properties that cannot beinferred from DNA or RNA sequencing alone, including associations between DNA copy numbervariation and gene expression dosage. We further demonstrate the detection of coding interchromosomalfusions and single nucleotide variants in both the genomes and transcriptomes ofindividual cells. G&T-seq enables the study of genotype-phenotype associations in single cells, andthe investigation of DNA cell lineage trees of healthy and diseased tissues with transcriptome inferredcell types and states.

Study Datasets 1 dataset.

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Dataset ID Description Technology Samples
Development of a method for separation and parallel sequencing of the genomes and transcriptomes of single cells.
HiSeq X Ten,Illumina HiSeq 2500,Illumina MiSeq 700

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