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G&T-seq: parallel sequencing of the genomes and transcriptomes of single cells

The ability to simultaneously sequence the genome and transcriptome of the same single cell offersa powerful means to dissect functional genetic heterogeneity at the cellular level. Here we describeG&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from singlecells. By applying G&T-seq to over 220 single cells we reveal cellular properties that cannot beinferred from DNA or RNA sequencing alone, including associations between DNA copy numbervariation and gene expression dosage. We further demonstrate the detection of coding interchromosomalfusions and single nucleotide variants in both the genomes and transcriptomes ofindividual cells. G&T-seq enables the study of genotype-phenotype associations in single cells, andthe investigation of DNA cell lineage trees of healthy and diseased tissues with transcriptome inferredcell types and states.

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID Description Technology Samples
EGAD00001001332 HiSeq X Ten Illumina HiSeq 2500 Illumina MiSeq 700
Publications Citations
G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.
Nat Methods 12: 2015 519-522
374
Power analysis of single-cell RNA-sequencing experiments.
Nat Methods 14: 2017 381-387
273
Integrative Methods and Practical Challenges for Single-Cell Multi-omics.
Trends Biotechnol 38: 2020 1007-1022
81