Study

Leucocyte eQTLs in autoimmune disease and health

Study ID Alternative Stable ID Type
EGAS00001001251 Other

Study Description

Peripheral blood was taken from healthy controls and patients with active autoimmune disease. CD4 T cells, CD8 T cells, monocytes, neutrophils, and B cells were separated using magnetic-activated cell sorting. RNA and genomic DNA was extracted from cell lysates using Qiagen Allprep kits. Genotyping of healthy controls and patients with inflammatory bowel disease was performed using the Illumina Human OmniExpress12v1.0 BeadChip at the Wellcome Trust Sanger Institute. 200 ng RNA was processed for hybridization onto Affymetrix Human Gene ST 1.1 microarrays. Gene expression and genotyping data was used to perform eQTL mapping.

Study Datasets 1 dataset.

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Dataset ID Description Technology Samples
EGAD00010000748
Genotyping using Illumina Human OmniExpress12v1.0
1

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