Study

An integrative model of pathway convergence in genetically heterogeneous blast crisis chronic myeloid leukemia (CML)

Study ID Alternative Stable ID Type
EGAS00001001751 Other

Study Description

Targeted therapies against the BCR-ABL1 kinase have revolutionized treatment of chronic phase (CP) chronic myeloid leukemia (CML). In contrast, management of blast crisis (BC) CML remains challenging because BC cells acquire complex molecular alterations that confer stemness features to progenitor populations and resistance to BCR-ABL1 tyrosine kinase inhibitors. Comprehensive models of BC transformation have proved elusive because of the rarity and genetic heterogeneity of BC, but are important for developing biomarkers predicting BC progression and effective therapies. To better understand BC, we performed an integrated multiomics analysis of 74 CP and BC samples using whole-genome and exome sequencing, transcriptome and methylome profiling, and chromatin immunoprecipitation followed by high-throughput sequencing. Employing pathway-based analysis, we found the BC genome was significantly enriched for mutations affecting components of the polycomb repressive complex (PRC) pathway. While transcriptomically, BC progenitors were enriched and depleted for PRC1- and PRC2-related gene ... (Show More)

Study Datasets 12 datasets.

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID Description Technology Samples
EGAD00001002053
dataset CML WGS VCF
29
EGAD00001002054
dataset CML WES VCF
24
EGAD00001002057
dataset CML WGS pairend fastq
Illumina HiSeq 2000 29
EGAD00001002058
dataset CML WGS pairend bam
HiSeq X Ten,Illumina HiSeq 2000 33
EGAD00001002059
dataset CML WES pairend fastq
Illumina HiSeq 2000 24
EGAD00001002060
dataset CML WES pairend bam
Illumina HiSeq 2000 24
EGAD00001002061
BMI1 ChIP-seq on human K562
Illumina HiSeq 2500 3
EGAD00001002062
BMI1 ChIP-seq on human K562
Illumina HiSeq 2500 3
EGAD00010002209
Expression dataset for CD34 sorted primary CML bone marrow samples
Illumina Beadchip HT12v4 34
EGAD00010002210
Expression dataset for DAC+PTC209 treated CD34 sorted primary CML bone marrow samples
Illumina Beadchip HT12v4 44
EGAD00010002211
Expression dataset for DAC treated CD34 sorted primary CML bone marrow samples
Illumina Beadchip HT12v4 48
EGAD00010002216
DNA methylation array from primary samples
Illumina 450K 65

Who archives the data?

There are no publications available