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Whole genome sequencing of colon organoid cultures with artificially induced oncogenic mutations

Using the CRISPR-Cas9 genome editing system, various oncogenic mutations were introduced in the genome of normal adult stem cells derived from the colon. The resulting clonal cultures were subjected to whole genome sequencing. In addition, the cultures were transplanted in a mouse and the resulting primary and metastatic tumors were also subjected to whole genome sequencing. The initial bulk culture, which was used to generate the mutations, was also whole genome sequenced and served as a reference sample to filter germline variants.

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID Description Technology Samples
EGAD00001002719 HiSeq X Ten 30
Publications Citations
The somatic POLE P286R mutation defines a unique subclass of colorectal cancer featuring hypermutation, representing a potential genomic biomarker for immunotherapy.
Oncotarget 7: 2016 68638-68649
Genetic dissection of colorectal cancer progression by orthotopic transplantation of engineered cancer organoids.
Proc Natl Acad Sci U S A 114: 2017 E2357-E2364
Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer.
Science 358: 2017 234-238