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Integrative single-cell and cell-free plasma RNA transcriptomics elucidates placental cellular dynamics

The human placenta is a dynamic and cellular heterogeneous organ, which is critical in fetomaternal homeostasis and the development of preeclampsia. Previous work has shown that placenta-derived cell-free RNA increases during pregnancy. We applied large-scale microfluidic single-cell transcriptomic technology to comprehensively characterize cellular heterogeneity of the human placentas and identified multiple placental cell-type–specific gene signatures. Analysis of the cellular signature expression in maternal plasma enabled noninvasive delineation of the cellular dynamics of the placenta during pregnancy and the elucidation of extravillous trophoblastic dysfunction in early preeclampsia.

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Dataset ID Description Technology Samples
EGAD00001003705 Illumina HiSeq 2000 NextSeq 500 32
Publications Citations
Integrative single-cell and cell-free plasma RNA transcriptomics elucidates placental cellular dynamics.
Proc Natl Acad Sci U S A 114: 2017 E7786-E7795
Current approaches and developments in transcript profiling of the human placenta.
Hum Reprod Update 26: 2020 799-840
MAAPER: model-based analysis of alternative polyadenylation using 3' end-linked reads.
Genome Biol 22: 2021 222
Transcriptomic Drivers of Differentiation, Maturation, and Polyploidy in Human Extravillous Trophoblast.
Front Cell Dev Biol 9: 2021 702046
Human placental biology at single-cell resolution: a contemporaneous review.
BJOG 129: 2022 208-220
The Function of the Kynurenine Pathway in the Placenta: A Novel Pharmacotherapeutic Target?
Int J Environ Res Public Health 18: 2021 11545
A cost-effective machine learning-based method for preeclampsia risk assessment and driver genes discovery.
Cell Biosci 13: 2023 41
Histopathologic and Transcriptomic Profiling Identifies Novel Trophoblast Defects in Patients With Preeclampsia and Maternal Vascular Malperfusion.
Mod Pathol 36: 2023 100035
Placental cell type deconvolution reveals that cell proportions drive preeclampsia gene expression differences.
Commun Biol 6: 2023 264