Single cell exome sequencing of lung adenocarcinoma

Study ID Alternative Stable ID Type
EGAS00001002972 Other

Study Description

Single cell exome sequencing was performed on 200 single cells isolated from a lung adenocarcinoma, female, non-smoker patient. The cells were derived from two distant tumour sectors and a far normal sector. The single cells were amplified on the Fluidigm C1 machine using the GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit prior to sequencing library preparation. In addition, bulk exome sequencing was also conducted for each tumour sectors. For this study, somatic variations were detected from the single cells and phylogenetic clustering was conducted to elucidate the evolutionary trajectory of lung cancer.

Study Datasets 1 dataset.

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Dataset ID Description Technology Samples
Illumina HiSeq 2000 195

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