Whole genome amplification and whole genome sequencing of human single cells

Human single fibroblasts and T-lymphocytes were amplified using multiple annealing and looping-based amplification and multiple displacement amplification. Amplified products were next subjected to whole genome sequencing with the goal of performing a genetic variant analysis.

Type: Other
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 2 Publications

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD00001004195	The dataset includes paired end fastq files of whole genome sequencing data on the Illumina platfrom. Individual samples are multiple annealing and looping based amplified single fibroblasts and multiple displacement amplified single T lympocytes, including unamplified bulk samples.	HiSeq X Ten Illumina HiSeq 2500	36

Publications	Citations
Conbase: a software for unsupervised discovery of clonal somatic mutations in single cells through read phasing. Hård J, Al Hakim E, Kindblom M, Björklund ÅK, Sennblad B, Demirci I, Paterlini M, Reu P, Borgström E, Ståhl PL, Michaelsson J, Mold JE, Frisén J. Genome Biol 20: 2019 68	14
Scuphr: A probabilistic framework for cell lineage tree reconstruction. Koptagel H, Jun SH, Hård J, Lagergren J. PLoS Comput Biol 20: 2024 e1012094	2