Organoid Models of Human Liver Cancers Derived from Tumor Needle Biopsies

Study ID Alternative Stable ID Type
EGAS00001003115 Other

Study Description

Hepatocellular carcinoma (HCC) is the most common primary liver cancer and the second cause of cancer-related mortality worldwide. HCC mainly arises in livers with pre-existing underlying diseases including viral hepatitis, alcoholic liver disease and nonalcoholic steatohepatitis. The multikinase inhibitor Sorafenib is the only drug available for the treatment of advanced HCC. However, due to the different backgrounds and the resulting heterogeneity of tumors, its efficacy greatly varies between patients and is further limited due to adverse effects and the development of drug resistance. Current in vitro models to study HCC based on pre-existing cancer cell lines fail in recapitulating key features such as tumor architecture, cellular heterogeneity and cell-cell interactions. In this study we report the generation of long-term three-dimensional organoid cultures from tumor biopsies of HCC patients with different etiologies and tumor stages. We demonstrate that HCC organoids retain the morphology and histological grading of the primary tumor as well as the expression pattern of HCC ... (Show More)

Study Datasets 2 datasets.

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Dataset ID Description Technology Samples
Whole-exome sequencing was performed from organoids derived from 10 liver cancer biopsies (7 hepatocellular carcinoma and 3 cholangiocarcinoma), corresponding liver and non-tumoral biopsies. For 3 of the organoids, both early and late passage organoids were sequenced. Whole-exome sequencing was performed using the Agilent Clinical Research Exome capture kit followed by Illumina sequencing. BAM files are provided in this dataset.
Illumina HiSeq 2500 31
RNA-seq of Bone Metastasis from breast and prostate cancer (4 breast and 5 prostate samples). Dataset contains BAM files from RNA-seq performed using Illumina HiSeq 2500.
Illumina HiSeq 2500,Illumina NovaSeq 6000,Ion Torrent S5 XL 288

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