Study
Clonal Decomposition and DNA Replication States Defined by Scaled Single-Cell Genome Sequencing
Study ID | Alternative Stable ID | Type |
---|---|---|
EGAS00001003190 | Other |
Study Description
Accurate measurement of clonal genotypes, mutational processes, and replication states from individual tumor-cell genomes will facilitate improved understanding of tumor evolution. We have developed DLP+, a scalable single-cell whole-genome sequencing platform implemented using commodity instruments, image-based object recognition, and open source computational methods. Using DLP+, we have generated a resource of 51,926 single-cell genomes and matched cell images from diverse cell types including cell lines, xenografts, and diagnostic samples with limited material. From this resource we have defined variation in mitotic mis-segregation rates across tissue types and genotypes. Analysis of matched genomic and image measurements revealed correlations between cellular morphology and genome ploidy states. Aggregation of cells sharing copy number profiles allowed for calculation of single-nucleotide resolution clonal genotypes and inference of clonal phylogenies and avoided the limitations of bulk deconvolution. Finally, joint analysis over the above features defined clone-specific ... (Show More)
Study Datasets 63 datasets.
Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data
Dataset ID | Description | Technology | Samples |
---|---|---|---|
EGAD00001004553 |
Direct library preparation+ single-cell DNA-sequencing of (i) patient derived triple negative breast cancer xenograft (ii) primary tumour and ascites ovarian cancer cell lines at tumour recurrence.
|
Illumina HiSeq 2500 | 980 |
EGAD00001004719 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 1340 samples; filetype=bam
|
Illumina HiSeq 2500 | 1340 |
EGAD00001004720 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 2057 samples; filetype=bam
|
Illumina HiSeq 2500 | 2057 |
EGAD00001004721 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 1970 samples; filetype=bam
|
Illumina HiSeq 2500 | 1970 |
EGAD00001004722 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 2091 samples; filetype=bam
|
Illumina HiSeq 2500 | 2091 |
EGAD00001004723 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 1267 samples; filetype=bam
|
Illumina HiSeq 2500 | 1267 |
EGAD00001004724 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 230 samples; filetype=bam
|
Illumina HiSeq 2500 | 230 |
EGAD00001004725 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 232 samples; filetype=bam
|
Illumina HiSeq 2500 | 232 |
EGAD00001004726 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 239 samples; filetype=bam
|
Illumina HiSeq 2500 | 239 |
EGAD00001004727 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 692 samples; filetype=bam
|
Illumina HiSeq 2500 | 692 |
EGAD00001004728 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 612 samples; filetype=bam
|
Illumina HiSeq 2500 | 612 |
EGAD00001004729 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 1700 samples; filetype=bam
|
Illumina HiSeq 2500 | 1700 |
EGAD00001004730 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 628 samples; filetype=bam
|
Illumina HiSeq 2500 | 628 |
EGAD00001004731 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 596 samples; filetype=bam
|
Illumina HiSeq 2500 | 596 |
EGAD00001004732 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 1735 samples; filetype=bam
|
Illumina HiSeq 2500 | 1735 |
EGAD00001004733 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 585 samples; filetype=bam
|
NextSeq 550 | 585 |
EGAD00001004734 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 766 samples; filetype=bam
|
NextSeq 550 | 766 |
EGAD00001004735 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 2055 samples; filetype=bam
|
Illumina HiSeq 2500 | 2055 |
EGAD00001004736 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 620 samples; filetype=bam
|
Illumina HiSeq 2500 | 620 |
EGAD00001004737 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 624 samples; filetype=bam
|
NextSeq 550 | 624 |
EGAD00001004738 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 481 samples; filetype=bam
|
NextSeq 550 | 481 |
EGAD00001004739 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 378 samples; filetype=bam
|
Illumina HiSeq 2500 | 378 |
EGAD00001004740 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 735 samples; filetype=bam
|
Illumina HiSeq 2500 | 735 |
EGAD00001004741 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 718 samples; filetype=bam
|
Illumina HiSeq 2500 | 718 |
EGAD00001004742 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 493 samples; filetype=bam
|
NextSeq 550 | 493 |
EGAD00001004743 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 1222 samples; filetype=bam
|
Illumina HiSeq 2500 | 1222 |
EGAD00001004744 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 522 samples; filetype=bam
|
Illumina HiSeq 2500 | 522 |
EGAD00001004745 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 488 samples; filetype=bam
|
Illumina HiSeq 2500 | 488 |
EGAD00001004746 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 509 samples; filetype=bam
|
NextSeq 550 | 509 |
EGAD00001004747 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 604 samples; filetype=bam
|
NextSeq 550 | 604 |
EGAD00001004748 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 626 samples; filetype=bam
|
NextSeq 550 | 626 |
EGAD00001004749 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 635 samples; filetype=bam
|
Illumina HiSeq 2500 | 635 |
EGAD00001004750 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 1522 samples; filetype=bam
|
HiSeq X Five | 1522 |
EGAD00001004751 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 465 samples; filetype=bam
|
NextSeq 550 | 465 |
EGAD00001004752 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 606 samples; filetype=bam
|
Illumina HiSeq 2500 | 606 |
EGAD00001004753 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 615 samples; filetype=bam
|
NextSeq 550 | 615 |
EGAD00001004754 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 636 samples; filetype=bam
|
NextSeq 550 | 636 |
EGAD00001004755 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 968 samples; filetype=bam
|
HiSeq X Five | 968 |
EGAD00001004756 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 480 samples; filetype=bam
|
NextSeq 550 | 480 |
EGAD00001004757 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 561 samples; filetype=bam
|
NextSeq 550 | 561 |
EGAD00001004758 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 844 samples; filetype=bam
|
HiSeq X Five | 844 |
EGAD00001004759 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 928 samples; filetype=bam
|
HiSeq X Five | 928 |
EGAD00001004760 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 635 samples
|
HiSeq X Five | 635 |
EGAD00001004761 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 1072 samples
|
HiSeq X Five | 1072 |
EGAD00001004762 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 1436 samples; filetype=bam
|
HiSeq X Five | 1436 |
EGAD00001004763 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 589 samples; filetype=bam
|
HiSeq X Five | 589 |
EGAD00001004764 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 656 samples; filetype=bam
|
HiSeq X Five | 656 |
EGAD00001004765 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 648 samples; filetype=bam
|
HiSeq X Five | 648 |
EGAD00001004766 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 375 samples; filetype=bam
|
HiSeq X Five | 375 |
EGAD00001004767 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 755 samples; filetype=bam
|
HiSeq X Five | 755 |
EGAD00001004768 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 492 samples; filetype=bam
|
HiSeq X Five | 492 |
EGAD00001004769 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 531 samples; filetype=bam
|
HiSeq X Five | 531 |
EGAD00001004770 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 1222 samples; filetype=bam
|
HiSeq X Five | 1063 |
EGAD00001004771 |
Transposase-based amplification-free single-cell genome direct library preparation in nanowell chips; 522 samples; filetype=bam
|
HiSeq X Five,Illumina HiSeq 2500 | 742 |
EGAD00001005338 |
Whole genome sequencing of single cells identifies stochastic aneuploidies, genome replication, states, and clonal repertoires for library A96146A 1195 samples; filetype=bam
|
HiSeq X Five | 3 |
EGAD00001005340 |
Whole genome sequencing of single cells identifies stochastic aneuploidies, genome replication, states, and clonal repertoires for library A96172B 1694 samples; filetype=bam
|
HiSeq X Five | 3 |
EGAD00001005345 |
Whole genome sequencing of single cells identifies stochastic aneuploidies, genome replication, states, and clonal repertoires for library A96226B 1274 samples; filetype=bam
|
HiSeq X Five | 4 |
EGAD00001005347 |
Whole genome sequencing of single cells identifies stochastic aneuploidies, genome replication, states, and clonal repertoires for library A96193B 2410 samples; filetype=bam
|
HiSeq X Five | 3 |
EGAD00001005348 |
Whole genome sequencing of single cells identifies stochastic aneuploidies, genome replication, states, and clonal repertoires for library A96199A 843 samples; filetype=bam
|
HiSeq X Five | 3 |
EGAD00001005353 |
Whole genome sequencing of single cells identifies stochastic aneuploidies, genome replication, states, and clonal repertoires for library A96199B 1170 samples; filetype=bam
|
HiSeq X Five | 3 |
EGAD00001005354 |
Whole genome sequencing of single cells identifies stochastic aneuploidies, genome replication, states, and clonal repertoires for library A96211C 1397 samples; filetype=bam
|
HiSeq X Five | 3 |
EGAD00001005355 |
Whole genome sequencing of single cells identifies stochastic aneuploidies, genome replication, states, and clonal repertoires for library A96225C 1034 samples; filetype=bam
|
HiSeq X Five | 2 |
EGAD00001007756 |
OV2295-052021 dataset
|
Illumina HiSeq 2000 | 1 |
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