FBSeq: RNA sequencing of human fetal brain.

RNA sequencing of 120 human fetal brains (aged 12-19 post-conception weeks) was performed as part of a Medical Research Council (U.K.) funded project (MR/L010674/2) to investigate genetic effects on gene expression in the developing human brain and their role in neuropsychiatric disorders. Human fetal brain tissue from elective terminations of pregnancy was provided by the Human Developmental Biology Resource (HDBR) (http://www.hdbr.org), with ethical approval (#08/H0712/34), and with consent for use of fetal material for medical research provided by female donors. Total RNA was extracted from half of the available brain tissue from each fetus using Tri-Reagent and treated with TURBO DNase. RNA-Seq libraries were prepared using 1µg of purified total RNA and the Illumina TruSeq Stranded Total RNA Library Prep kit, following ribosomal RNA depletion. Libraries were sequenced on the Illumina HiSeq 2500 or HiSeq 4000 systems, generating at least 50 million read pairs per sample.

Type: Other
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 2 Publications

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD00001004363	FastQ files with paired-end RNAseq data for human fetal brain homogenate from 120 samples (12-19 post-conception weeks).	Illumina HiSeq 2500 Illumina HiSeq 4000	120

Publications	Citations
Expression quantitative trait loci in the developing human brain and their enrichment in neuropsychiatric disorders. O'Brien HE, Hannon E, Hill MJ, Toste CC, Robertson MJ, Morgan JE, McLaughlin G, Lewis CM, Schalkwyk LC, Hall LS, Pardiñas AF, Owen MJ, O'Donovan MC, Mill J, Bray NJ. Genome Biol 19: 2018 194	79
Cis-effects on gene expression in the human prenatal brain associated with genetic risk for neuropsychiatric disorders. Hall LS, Pain O, O'Brien HE, Anney R, Walters JTR, Owen MJ, O'Donovan MC, Bray NJ. Mol Psychiatry 26: 2021 2082-2088	16