Single cell analysis T cell activation
|Study ID||Alternative Stable ID||Type|
Study Datasets 2 datasets.
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We isolated naive and memory CD4+ T cells from 119 healthy individuals and stimulated the cells using anti-CD3/anti-CD28 coated beads. We profiled gene expression using single cell RNA-seq (10X-Genomics 3’ v2 kit) at resting state and three time points of activation (16h, 40h and 5 days post stimulation) and mapped expression quantitative trait loci.
|Illumina HiSeq 4000,Illumina MiSeq||167|
Blood samples were obtained from 119 healthy individuals of British ancestry. Genomic DNA was isolated from a suspension of PBMCs from each individual using a DNA isolation kit (Qiagen). Genotyping was then performed using the Infinium CoreExome-24 (v1.3) chip (Illumina).
|Infinium CoreExome-24 (v1.3) chip (Illumina)||127|