Single-cell Genomic and Transcriptomic Landscapes of Primary and Metastatic Tumors in Colorectal Cancer

Here we performed a single-cell RNA-Seq survey of 7,438 cells from 9 metastatic CRC patients, from whom matching adjacent normal tissues, primary tumors, and metastatic tumors were simultaneously sampled. Moreover, we also performed whole genome sequencing (WGS) on 81 sampled regions of these 9 patients and multi-region whole exome sequencing (WES) on 35 sample regions from 3 of these patients (patient #7-#9). We analyzed the dynamics of cell-type compositions, genomic copy number variants (CNVs), gene expression signatures, crosstalk between cancer cells and microenvironment cells and somatic mutations during CRC tumorigenesis and metastasis.

• Type: Other
• Archiver: European Genome-phenome Archive (EGA)