Study
Fresh vs. frozen cell preparations of colorectal cancer single-cell RNA sequencing
Study ID | Alternative Stable ID | Type |
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EGAS00001003769 | RNASeq |
Study Description
Study Datasets 1 dataset.
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Dataset ID | Description | Technology | Samples |
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EGAD00001005191 |
To evaluate 3 different tissue dissociation protocols or fresh vs. frozen cell preparations, we performed single-cell RNA sequencing on cancer or distant normal tissue dissociates from 2 colorectal cancer patients. Total 18,409 cells from 10 sample preparations were analyzed (5 primary colorectal cancer and 5 matched normal mucosa). The results suggest highly consistent cellular proportions were recovered with different sample preparation methods.
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Illumina HiSeq 2500 | 10 |
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