Study

Fresh vs. frozen cell preparations of colorectal cancer single-cell RNA sequencing

Study ID Alternative Stable ID Type
EGAS00001003769 RNASeq

Study Description

Study Datasets 1 dataset.

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Dataset ID Description Technology Samples
EGAD00001005191
To evaluate 3 different tissue dissociation protocols or fresh vs. frozen cell preparations, we performed single-cell RNA sequencing on cancer or distant normal tissue dissociates from 2 colorectal cancer patients. Total 18,409 cells from 10 sample preparations were analyzed (5 primary colorectal cancer and 5 matched normal mucosa). The results suggest highly consistent cellular proportions were recovered with different sample preparation methods.
Illumina HiSeq 2500 10

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