Gene_epression_regulation_in_cytokine_induced_immune_cell_states

We isolated T cells and monocotyes from healthy platelet donors and cultured them in resting and stimulated conditions with addition of a range of cytokines. We performed RNA sequencing to assess impact of different disease-relevant cytokines upon immune cell responses.

Type: Gene Regulation Study
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 2 Publications

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Dataset ID	Description	Technology	Samples
EGAD00001005291	Cytokines affect T cell responses by polarising them to different phenotypes. We isolated T cells from healthy platelet donors and cultured them in resting and stimulated conditions, as well as in the presence of Th1, Th2, Th17 and iTreg cocktail. In addition, T cells were stimulated in the presence of IL-10, IL-21, IL-27, IFNb and TNFa. We performed bulk RNA sequencing to assess impact of different disease-relevant cytokines upon T cell response.	Illumina HiSeq 2500	141

Publications	Citations
Single-cell transcriptomics identifies an effectorness gradient shaping the response of CD4<sup>+</sup> T cells to cytokines. Cano-Gamez E, Soskic B, Roumeliotis TI, So E, Smyth DJ, Baldrighi M, Willé D, Nakic N, Esparza-Gordillo J, Larminie CGC, Bronson PG, Tough DF, Rowan WC, Choudhary JS, Trynka G. Nat Commun 11: 2020 1801	114
Analyzing sex imbalance in EGA and dbGaP biological databases: Recommendations for better practices. Ruiz-Serra V, Buslón N, Philippe OR, Saby D, Morales M, Pontes C, Andirkó AM, Holliday GL, Jené A, Moldes M, Rambla J, Valencia A, Rementeria MJ, Cortés A, Cirillo D. iScience 27: 2024 110831	0