Study
Gene epression regulation in cytokine induced immune cell states
Study ID | Alternative Stable ID | Type |
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EGAS00001003823 | Gene Regulation Study |
Study Description
We isolated T cells and monocotyes from healthy platelet donors and cultured them in resting and stimulated conditions with addition of a range of cytokines. We performed RNA sequencing to assess impact of different disease-relevant cytokines upon immune cell responses.
Study Datasets 1 dataset.
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Dataset ID | Description | Technology | Samples |
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EGAD00001005291 |
Cytokines affect T cell responses by polarising them to different phenotypes. We isolated T cells from healthy platelet donors and cultured them in resting and stimulated conditions, as well as in the presence of Th1, Th2, Th17 and iTreg cocktail. In addition, T cells were stimulated in the presence of IL-10, IL-21, IL-27, IFNb and TNFa. We performed bulk RNA sequencing to assess impact of different disease-relevant cytokines upon T cell response.
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Illumina HiSeq 2500 | 141 |
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