Defective mitophagy and enhanced oxidative stress dictate regulatory T cell impairment in autoimmunity

Regulatory T cell (Treg) impairment is a common denominator of autoimmune pathologies. Despite the extensive characterization of Treg cell phenotype in autoimmunity, the molecular mechanisms underlying their deterioration remain ill defined. To investigate Treg dysfunction during autoimmunity we performed transcriptomic analysis of CD4+CD25highCD127- Treg cells from peripheral blood of patients with autoimmunity.

Type: Other
Archiver: European Genome-Phenome Archive (EGA)

2 Datasets 2 Publications

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Dataset ID	Description	Technology	Samples
EGAD00001005446	Tregs were sorted as CD4+CD25+CD127- cells from peripheral blood of 14 healthy individuals, 8 patients with mild/severe rheumatoid arthritis, 1 patient with systemic lupus erythematosus/rheumatoid arthritis, 2 patients with ulcerative colitis and 2 patients with Chrohn's disease. RNA was extracted and polyA libraries were prepared using the Illumina Truseq sample preparation kit v.2. Single-end 75bp sequencing was performed on NextSeq500.	NextSeq 500	27
EGAD00001006193	Tregs were sorted as CD4+CD25+CD127- cells from peripheral blood of 14 healthy individuals, 8 patients with systemic lupus erythematosus, 9 patients with rheumatoid arthritis, and 11 patients with multiple sclerosis. RNA was extracted and polyA libraries were prepared using the Illumina Truseq sample preparation kit v.2. Single-end sequencing was performed on NextSeq500.	NextSeq 500	42

Publications	Citations
Mitochondrial Oxidative Damage Underlies Regulatory T Cell Defects in Autoimmunity. Alissafi T, Kalafati L, Lazari M, Filia A, Kloukina I, Manifava M, Lim JH, Alexaki VI, Ktistakis NT, Doskas T, Garinis GA, Chavakis T, Boumpas DT, Verginis P. Cell Metab 32: 2020 591-604.e7	83
Dissecting the role of <i>CSF2RB</i> expression in human regulatory T cells. Côrte-Real BF, Arroyo Hornero R, Dyczko A, Hamad I, Kleinewietfeld M. Front Immunol 13: 2022 1005965	7