Prime editing for functional repair in patient-derived disease models
|Study ID||Alternative Stable ID||Type|
Study Datasets 1 dataset.
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We performed whole genome sequencing to detect possible off-target mutations induced by prime editing. Liver organoids, derived from a healthy control, were transfected with either control (GFP) plasmids or prime editing plasmids (GFP+PE2+pegRNA+nickRNA) to induce a 6-bp deletion in CTNNB1. One control and two prime-edited organoid lines were clonally expanded from single cells. High-throughput sequencing was performed on the complete genomic DNA isolated from these clonal lines, as well as the ... (Show More)
|Illumina NovaSeq 6000||8|