HCA_Kidney_Adult_WSSS_RNA

Defining regional anatomical variation in cellular composition, transcriptome and epigenome in the human kidney The kidneys play a critical role in homeostasis, excreting waste and acid and maintaining electrolyte and water balance. The homeostatic function of the kidney generates substantial differences in tissue environment between different anatomical regions of the kidney. Complementing efforts in generating single-cell molecular profiles across the entire cross-section of the adult human kidney, from outer cortex to upper ureter, we aim to also interrogate whether the epigenome of kidney-resident immune cells varies across different anatomical regions of the kidney. The expression of RNA transcripts is, in part, controlled by the state of chromatin, with closed structures sterically hindering the binding of transcriptional modulators. The chromatin landscape of immune cells can change according to environmental stimuli, for example, via engagement of toll-like receptor (TLR)4 with lipopolysaccharide. Here we wish to determine whether and how the environmental differences in cortex, medulla and pelvis might impact resident immune cell epigenome using single-cell ATAC-seq on the 10x platform This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

• Type: Transcriptome Analysis
• Archive: European Genome-phenome Archive (EGA)