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Leukemia stem cell containing fractions

ATAC-seq performed on leukemic stem cell fractions with different engraftment potential. Leukemic cells were sorted out of Acute Myeloid Leukemia patient samples based on CD34/CD38 status. Samples were annotated based on an independent engraftment assay in mice as LSC+ (engrafting) and LSC- (not engrafting). Omni-ATAC-seq was carried out to compute differential enrichment of transposable elements in chromatin accessible regions.

Type: Other
Archiver: European Genome-phenome Archive (EGA)

1 Dataset 1 Publication

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Dataset ID	Description	Technology	Samples
EGAD00001006775	AML bulk samples were sorted into fractions based on expression of CD34 and CD38, LSC activity in each fraction was assessed by xenotransplantation into NOD. Prkdcscid. Il2rgnull(NSG) mice. ATAC-seq was used to profile the accessible chromatin landscape of 11 LSC+, 24 LSC- fractions . 50,000 cells from each sample were processed. Libraries were sequenced with 50bp single-end reads.	unspecified	35

Publications	Citations
Transposable elements shape stemness in normal and leukemic hematopoiesis. Grillo G, Nadorp B, Qamra A, Drylie B, Mitchell A, Arlidge C, Nand A, Takayama N, Murison A, Madani Tonekaboni SA, Kang KK, Arruda A, Wang JCY, Minden MD, Deniz Ö, Boutzen H, Dick JE, Lupien M. Nat Genet 58: 2026 1087-1099	0