DNA methylation analysis on PBL obtained from male patients with UC-PSC, UC and HC

Study ID Alternative Stable ID Type
EGAS00001005832 Other

Study Description

Primary sclerosing cholangitis (PSC) is a chronic inflammatory liver disease affecting the intra- and extrahepatic bile ducts, and is strongly associated with ulcerative colitis (UC). In this study, we explored the peripheral blood DNA methylome and its immune cell composition in patients with PSC-UC, UC, and healthy controls (HC) with the aim to develop a predictive assay in distinguishing patients with PSC-UC from those with UC alone. The peripheral blood DNA methylome of male patients with PSC and concomitant UC, UC and HCs was profiled using the Illumina HumanMethylation Infinium EPIC BeadChip (850K) array. Differentially methylated CpG position (DMP) and region (DMR) analyses were performed alongside gradient boosting classification analyses to discern PSC-UC from UC patients. As observed differences in the DNA methylome could be the result of differences in cellular populations, we additionally employed mass cytometry to characterize the immune cell compositions. Genome wide methylation analysis revealed no differentially methylated positions between PSC-UC and UC patients. ... (Show More)

Study Datasets 1 dataset.

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Dataset ID Description Technology Samples
Whole-genome DNA methylation profiling of PBL obtained from male patients with PSC-UC, or UC alone, or healthy individuals.
Illumina Infinium HumanMethylation EPIC BeadChip 47

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