Comparison of fresh and slow-frozen cancer samples for different applications

Surplus tissues from multiple solid human cancers directly slow-frozen after resection can subsequently be used for different types of methods including the establishment of 2D, 3D, and ex vivo cultures as well as single cell RNA sequencing (scRNAseq) with similar results when compared to freshly analyzed material.

Type: Other
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD00001009284	Bam files of 17 samples from 11 different patients. The scRNAseq data were obtained using the 10X 3' Gene Expression kit.	Illumina NovaSeq 6000	17

Publications	Citations
Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseq. Restivo G, Tastanova A, Balázs Z, Panebianco F, Diepenbruck M, Ercan C, Preca BT, Hafner J, Weber WP, Kurzeder C, Vetter M, Soysal SM, Beisel C, Bentires-Alj M, Piscuoglio S, Krauthammer M, Levesque MP. Commun Biol 5: 2022 1144	3