An Isogenic Cell Line Panel for Sequence-based Screening of Targeted Anti-cancer Drugs

Study ID Alternative Stable ID Type
EGAS00001005974 Other

Study Description

We describe the creation and characterization of an isogenic cell line panel representing common cancer pathways, with multiple features optimized for high-throughput screening. More than 1,800 cell lines from three normal human cells were generated using CRISPR-technologies. Surprisingly, we discovered most of these lines did not result in complete gene inactivation, despite integration of sgRNA at the desired genomic site. However, a subset of the lines harbored true, biallelic disruptions of the targeted tumor suppressor gene, yielding a final panel of 100 well-characterize lines covering 19 pathways frequently subject to loss of function in cancers. This panel included genetic markers optimized for sequence-based ratiometric assays for drug-based screening assays. To illustrate the potential utility of this panel, we developed a multiplexed high-throughput screen that identified Wee1 inhibitor MK-1775 as a selective growth inhibitor of cells with inactivation of TP53. These cell lines and screening approach should prove useful for researchers studying a variety of cellular and ... (Show More)

Study Datasets 1 dataset.

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Dataset ID Description Technology Samples
Libraries were prepared from RNA-extracted cell lines using Illumina RNA library prep kit. Samples were sequenced on Illumina HiSeq 4000 or HiSeq 2500.
Illumina HiSeq 2500,Illumina HiSeq 4000 103

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