Bulk RNA sequencing of Singapore colorectal cancer patients (SG-BULK)
|Study ID||Alternative Stable ID||Type|
The study was approved by the institutional review board of Singhealth (2018-2795). Ten 5-µm tissue sections were cut using standard microtomy techniques. Each collected cell population was then extracted using the allprep kit. Extracted material was then quantified using a Qubit fluorometer.
Study Datasets 1 dataset.
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RNA-seq libraries were prepared using the KAPA Stranded RNA-Seq Kit with RiboErase (Kapa Biosystems, Wilmington, MA) and sequenced to a target depth of 200-M reads on the Illumina HiSeq platform (Illumina, San Diego, CA).
|Illumina HiSeq 4000||162|
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