Study

Plasma whole genome sequencing from patients with stage IV colorectal cancer and microsatellite instability

Study ID Alternative Stable ID Type
EGAS00001006377 Other

Study Description

This study aims to explore genomic features of cancer in plasma WGS data from patients with advanced colorectal cancer. Cell-free DNA (cfDNA) was extracted from plasma using the QIAamp Circulating Nucleic Acid Kit. Libraries were prepared with 5 to 250 ng of cfDNA using the NEBNext DNA Library Prep Kit.

Study Datasets 1 dataset.

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Dataset ID Description Technology Samples
EGAD00001008999
This dataset contains plasma WGS data from patients with stage IV colorectal cancer (CRC, n = 16) and healthy individuals (n = 21) used in the Pointy manuscript. Patients with CRC provided written consent and samples were collected as performed as described previously (Clinical-Trials.gov number NCT01876511; Georgiadis et al., 2019, Le et al., 2017). Plasma samples from 21 healthy control individuals were procured through BioIVT. Cell-free DNA (cfDNA) was extracted from plasma using the QIAamp ... (Show More)
Illumina HiSeq 2000 37

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