Identification of neoantigen from MSI-CRC patient derived organoids and its application for targeting by autologous T cells

Study ID Alternative Stable ID Type
EGAS00001006633 Other

Study Description

Targeting neoantigens expressed only by tumor cells enable tumor destruction without unexpected damages to healthy tissues. Here, we identified neoantigens from microsatellite instable (MSI) colorectal cancer (CRC) patient-derived organoids by genomics and immunopeptidomics approches. The neoantigens in this study were predicted from frame-shift mutation by whole-genome sequencing and confirmed its presentation on organoids surface class I HLA molecules by mass spectrometry. We utilized these neoantigens to isolate neoantigen-specific CD8 T cells from autologous PBMC, and proved tumor organoids killing ability of the CD8 T cells, whereas it did not attack normal organoids. This study provides a platform to identify neoantigen from patient-derived cancer organoids and a model to validate T cell recognition, which could be useful for adoptive CD8 T cell transfer treatment for MSI-CRC patient.

Study Datasets 1 dataset.

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Dataset ID Description Technology Samples
Contains 4 clonal organoid samples + 1 bulk healthy tissue sample
HiSeq X Ten 5

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